The use of cotyledonary-node explants in Agrobacterium tumefaciensmediated transformation of cucumber (Cucumis sativus L.)
نویسندگان
چکیده
منابع مشابه
Agrobacterium-Mediated Transformation in Cucumber (Cucumis sativus L.)
Introduction. Cucumber (Cucumis sativus L.) is an important horticultural crop. Its fruits are used for slicing and pickling and juice extraction worldwide. In India, both the fruits and seeds have long been used in the manufacture of traditional medicines that act as skin conditioners, diuretics and body coolants. Thus, cucumber is a potential candidate for an edible vaccine (6). In India, stu...
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A reproducible and highly efficient protocol for genetic transformation mediated by Agrobacterium has been established for greengram (Vigna radiata L. Wilczek). Double cotyledonary node (DCN) explants were inoculated with Agrobacterium tumefaciens strain LBA 4404 harboring a binary vector pCAMBIA 2301 containing neomycin phosphotransferase (npt II) gene as selectable marker, β-glucuronidase (GU...
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In this research, twenty genotypes of cucumber (Cucumis sativus) were studied that were collected from different geographical regions. The experiment was conducted as a randomized complete Block Design (RCBD) with four replications in Greenhouse. Data was collected on morphological features, yield and components of yield of cucumber which include: Total fruit yield per pickling, Fruit number pe...
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Ethyl methanesulfonate (EMS) is a stable and effective chemical mutagen. In this study, cucumber (Cucumis sativus L. cv. “Shannong No. 5”) seeds were treated by 1% EMS for 12 h, 24 h and 48 h to optimize EMS mutagenesis and determined median lethal dose of EMS (1% EMS and 24 h) for “Shannong No. 5”. After treated by 1% EMS for 24 h, 541 M1 plants were grown in greenhouse for phenotype investiga...
متن کاملPolymorphom of sexually different cucumber (Cucumis sativus L.) NIL lines.
Isolations of polymorphic sequences of two pairs of the NIL lines of cucumber (Cucumis sativus L.), which differ with respect to sex, were carried out using the subtraction hybridization methods of DSC (Differential Subtraction Chain) and GDDSC (Genetically Directed DSC). 266 DSC tags were isolated from the entire genome region, and 38 GDDSC tags were isolated from the region containing the sex...
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ژورنال
عنوان ژورنال: Journal of Plant Biotechnology
سال: 2011
ISSN: 1229-2818
DOI: 10.5010/jpb.2011.38.3.198